Essential genes detection with Transposon insertion sequencing

Essential genes detection with Transposon insertion sequencing

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Delphine Lariviere, Bérénice Batut, Essential genes detection with Transposon insertion sequencing (Galaxy Training Materials). https://training.galaxyproject.org/training-material/topics/genome-annotation/tutorials/tnseq/tutorial.html Online

Abstract

Questions: What is Transposon insertion Sequencing? How to get TA sites Coverage? How to predict essential genes? Objectives: Understand the read structure of TnSeq analyses, Predict Essential genes with Transit, Compare gene essentiality in control sample and an different experimental condition

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